TDValidator
Our deep dive software for targeted proteoform analysis.
A robust machine for proteoform analysis.
With TDValidator, you can perform robust targeted analysis of a single proteoform or multiple proteoforms.
Get TDValidator
TDValidator is available for purchase through our customer service. To request a free 30-day demo or a purchase quote, contact us. Academic pricing is available.
Analyze.
Perform targeted analysis of a single proteoform or multiple proteoforms.
Input proteoforms for a single fragment spectrum and match all of the theoretical fragment ions to the spectral data.
Match precursor ions to precursor spectral data.
Perform fragmentation matching on data from a wide range of fragmentation techniques, including CID, HCD, ETD, ECD, EThcD, ETciD, and UVPD.
Visualize.
Characterize proteoforms using fragmentation evidence.
Analyze fragmentation spectra and corresponding matching ions to confidently localize proteoform modifications.
Overlay matching ions from different proteoforms on the same spectra and uses a host of visualization tools and metrics to power in-depth study of proteoforms.
Look for internal fragment ions formed during fragmentation processes. The ions can be visualized by color gradient heat map on the fragment map itself. Internal fragment ions can provide additional evidence for modification site localization, increasing confidence in assignments.
Validate.
Assess fragmentation coverage using fragmentation maps and related metrics.
Validate fragmentation ion matches to confirm post-translational modification site assignments.
Research Highlights
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Accurate Sequence Analysis of a Monoclonal Antibody by Top-Down and Middle-Down Orbitrap Mass Spectrometry Applying Multiple Ion Activation Techniques
Luca Fornelli, Kristina Srzentić, Romain Huguet, Christopher Mullen, Seema Sharma, Vlad Zabrouskov, Ryan T. Fellers, Kenneth R. Durbin, Philip D.Compton, and Neil L. Kelleher
Anal. Chem., 2018; 90, 14, 8421–8429.
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Structural Analysis of Monoclonal Antibodies by Ultrahigh Resolution MALDI In-Source Decay FT-ICR Mass Spectrometry
Yuri E.M. van der Burgt, David P. A. Kilgour, Yury O. Tsybin, Kristina Srzentić, Luca Fornelli, Alain Beck, Manfred Wuhrer, and Simone Nicolardi
Anal. Chem., 2019; 91, 3, 2079–2085.
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Native top-down mass spectrometry provides insights into the copper centers of membrane-bound methane monooxygenase
Soo Y. Ro, Luis F. Schachner, Christopher W. Koo, Rahul Purohit, Jonathan P. Remis, Grace E. Kenney, Brandon W. Liauw, Paul M. Thomas, Steven M. Patrie, Neil L. Kelleher, and Amy C. Rosenzweig
Nat Comms., 2019; 10, 2675.