ProSight Lite
Your go-to fragment matching tool for single proteoforms.
This is fragment matching made easy.
Top-down experiments often focus on identifying and localizing post-translational modifications and other sources of mass shift on a known protein sequence. With ProSight Lite, you can match a single candidate sequence and its modifications against a set of mass spectrometric observations and produce publication-ready fragment maps.
Read more from the technical brief on ProSight Lite published in Proteomics.
Get ProSight Lite
ProSight is available for free as web application and a Windows desktop application. Choose the option that best suits your needs.
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ProSight Lite is available for free download through the National Resource for Translational and Developmental Proteomics.
Clicking the ProSight Lite Desktop button will bring you to the Installer page for latest version of ProSight Lite, where you will find more details, including installation requirements, tutorials, and updates.
Match.
Match experimentally-derived fragment ion lists to theoretical fragment ions of a proteoform.
Add modifications to proteoforms using a simple, straightforward interface.
Calculate confidence metrics such as a P-score and proteoform characterization score.
Visualize.
Visualize fragmentation matches using fragment flags to show N- and C- terminal fragmentation ions. Fragment flag colors correspond to the type of ion found. Post-translational modifications are color-coded for easy differentiation of modification type.
Export fragmentation maps as PNG or SVG for publication.