**Latest Release** ProSightPD 3.0: Fully Integrated Top Down Proteomics Search in Proteome Discoverer

ProSightPD 3.0 is our newest plug-in for Thermo Fisher’s Proteome Discoverer 2.4 application that allows running ProSight searches of top down and middle down data within the Proteome Discoverer environment. ProSightPD 3.0 includes the workflows and nodes needed to conduct absolute mass, biomarker, and gene-restricted searches from within the Proteome Discoverer interface and then to integrate these results with other PD nodes. In addition to ProSight Lite, ProSightPD 3.0 now also has study visualization capabilities through TDViewer as well as individual proteoform interrogation through TDValidator. All three of these external tools launch directly from ProSightPD for a seamless data analysis workflow. ProSightPD 3.0 includes High Mass for Medium-High and Low-High workflows analyzing proteins >30 kDa. Contact your local Thermo Sales representative for details on purchasing or upgrading to ProSightPD 3.0!

Please download the Proteome Discoverer 2.4 demo from Thermo’s software portal. A 60 day demo of ProSightPD 3.0 is included in the 3rd party installer.

Download TDValidator from our website and contact us for a demo license.

Contact Proteinaceous

Download TDValidator

TDViewer can be downloaded from the NRTDP website.

Download TDViewer

Description

Key Features of ProSightPD 3.0 include:

Easy viewing of Proteins, Isoforms, Proteoforms, and PRSMs.
Graphical top down workflow construction and integration with other Proteome Discoverer workflows.
Annotated proteoform (absolute mass), subsequence search (biomarker), and gene-restricted annotated proteoform and subsequence search types.
TDViewer integration allows for robust visualization of proteomics results.
Viewing spectral support with Proteome Discoverer Spectral View.
New fragmentation maps for visualization of MS/MS characterization, including combination fragment maps to assess fragmentation from different scans.
Protein Center information on identified proteins.
Direct export of Top Down results for use in other applications such as ProSight Lite and TDValidator.
Support for two new fragmentation methods: EThcD and UVPD.
PTCR and FAIMS support.
Multi-threading for fast processing.
High mass processing is included in the form of the ReSpect algorithm.
ProSightPD supports the following MS2 experiments: LO-HI, MEDIUM-HI, and HI-HI. Algorithms are included to perform analysis of isotopically-resolved data as well as charge state deconvolution.