The Blood Proteoform Atlas published in Science.

Photo by FLY:D on Unsplash.

A research team led by the Kelleher Research Group at Northwestern University has recently published the Blood Proteoform Atlas (BPA), mapping more than 56,000 unique proteoforms in 21 different cell types found in the blood — almost 10 times more than previous studies have shown. Using the BPA as a reference, the researchers worked with Josh Levitsky, M.D., a transplant hepatologist at Northwestern Medicine, to examine proteoforms in patient blood samples and identified a panel of 24 biomarkers that could be used to predict liver transplant rejection. The study, which is published in Science, marks a major milestone in the quest to chart the entire Human Proteome and  exemplifies how precision top-down proteomics can be used in the clinic.

The BPA uses proteoform results obtained from the annotated proteoform and subsequence search components of our ProSight search engine. Members of the Proteinaceous team— Joseph Greer, Ryan Fellers, and Matthew Robey— contributed to the study. We look forward to helping chart more of the human proteoform in the future! 

Read the full-length publication in Science (27 Jan 2022): The Blood Proteoform Atlas: A reference map of proteoforms in human hematopoietic cells.

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“ProSight Annotator: Complete control and customization of protein entries in UniProt xml files” published in Proteomics!